Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays
نویسندگان
چکیده
منابع مشابه
Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays.
A quantitative hepatitis C virus reverse transcriptase PCR (HCV RT-PCR) assay established in our laboratory was compared with the Roche Amplicor HCV Monitor test kit for agreement of test results and intra-assay variability. Both assays rely on reverse transcription and amplification of extracted RNA from patients' sera together with an internal RNA standard derived from the 5'-noncoding region...
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Hepatitis E virus (HEV) is a major cause of acute viral hepatitis in people in many developing countries and is also endemic in many industrialized countries. Mammalian HEV (mHEV) isolates can be divided into at least four recognized major genotypes. Several nucleic acid amplification techniques have been developed for mHEV detection, with great differences in sensitivity. The aim of this study...
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5 a Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary 6 Medicine, Iowa State University, Ames, Iowa, USA 7 b Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária 8 Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Brazil 9 Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine ...
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Hepatitis C virus (HCV) RNA qualitative and quantitative second generation assays (Amplicor HCV v2.0 and Amplicor HCV Monitor v2.0, respectively) were evaluated by testing serum samples from 132 blood donors anti-HCV positive HCV RNA negative by first generation qualitative assay and 326 viremic patients. An HCV RNA transcript was synthesized and ten-fold dilutions were used to assess sensitivi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.2.261-264.1996